Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 327
About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Advertise Login 
Year : 2015  |  Volume : 4  |  Issue : 2  |  Page : 109-114

Assessment of methemoglobin concentration, serum nitrate, and nitrite levels and their interrelationships with antioxidant status in the cord blood of neonates born via normal delivery versus neonates delivered by cesarean section in an Indian population

1 Department of Biochemistry, Calcutta National Medical College and Hospital, Malda, West Bengal, India
2 Department of Biochemistry, Malda Medical College and Hospital, Malda, West Bengal, India
3 Department of Gynaecology and Obstetrics, IPGMER and SSKM Hospital, Kolkata, West Bengal, India

Correspondence Address:
Dr. Manali Sinharay
Department of Biochemistry, Calcutta National Medical College and Hospital, 32, Gorachand Road, Kolkata - 700 014, West Bengal
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2249-4847.154105

Rights and Permissions

Background: Oxidative and nitrosative stress are known to be associated with the transition from fetal to neonatal life at birth, when newborns having a compromised antioxidant defense system are suddenly exposed to a normoxic environment (from a hypoxic state). The current study explores the levels of antioxidants, markers of oxidative and nitrosative stress, in the cord blood samples of neonates born by cesarean section (CS) and normal delivery (ND), to assess the effect that the mode of delivery has on neonates, as far as oxidative and nitrosative stress are concerned. Aims: The main purpose of this study was to compare the levels of oxidative stress, nitrosative stress, and the antioxidant status in the cord blood of neonates delivered by ND, versus neonates delivered by CS. Materials and Methods: Cord blood was collected from 80 full-term, mature neonates of both sexes; 40 born via ND and 40 delivered by elective CS under regional (spinal) anesthesia. The samples were tested for the percent of methemoglobin as a marker of oxidative stress, as well as the antioxidants red blood cell (RBC) glucose-6-phosphate dehydrogenase (G6PD), RBC reduced glutathione (GSH), and serum nitrate and nitrite as markers of nitrosative stress. The statistical evaluation of the data obtained was done using SPSS 17 software, taking the significance level as P < 0.05. Results: The comparison of the analytes between the two groups revealed that the percent of methemoglobin (P < 0.001) was significantly higher, and both RBC G6PD (P < 0.001) and RBC reduced GSH (P = 0.003) were significantly lower in CS neonates than in ND neonates. There were no significant differences in the serum nitrate (P = 0.273) and serum nitrite (P = 0.060) between the two groups of neonates. Conclusion: The CS neonates were deficient in antioxidants and had higher levels of oxidative stress than the ND neonates, but no significant difference existed in the nitrosative stress between the groups.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded207    
    Comments [Add]    

Recommend this journal